ANNEX 6: Cell Culture Room

General Information

Contact Person: Simon Henriet

The Sars Centre's guidelines are based on the National Institutes of Health's publication "Biosafety in Microbiological and Biomedical Laboratories", known as BMBL. For more information please reference http://www.cdc.gov/biosafety/publications/bmbl5/index.htm

Brief guidelines for working with cell cultures

Only authorised personnel are allowed to use the room. Group Leaders are responsible for ensuring the training and authorisation of lab members. For detailed instructions regarding safety, rules and best practices in the cell culture room, please refer to the facility handbook.

Brief guidelines for working with retroviruses

Viruses can be used for gene delivery into mammalian cell culture. The viruses currently used at the Sars Centre are not considered pathogenic (they can potentially infect human cells and integrate their DNA, but they cannot proliferate).