ANNEX 1: Quick Lab Safety Introduction List

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Item

Description

1 Knowledge of security item locations – first aid kits, eye rinsing bottles, emergency showers, fire-extinguishing equipment, emergency exits and meeting points – is an individual responsibility. Instructions will be given for NOKAS personal alarms.
2 All accidents, near-accidents or abnormalities must be reported to the Head of Administration and the Safety Officer (for safety, statistical, medical, insurance and prevention purposes).
3 Eating, drinking and smoking is strictly prohibited in the lab, smoking is prohibited (by law) inside the building.
4 Use of lab coats in the laboratories is compulsory because the Sars Centre’s Research involves Genetically Modified Organisms (GMO/GMM); the laboratories are classified as Risk Category II.
5 Always use gloves and wear a lab coat to protect yourself when working with hazardous materials.
  • Lab coats are to be worn while working in labs or moving between labs and common equipment areas.
  • Never wear lab coats and gloves in offices, the lunchroom, administrative areas or bathroom facilities.
  • When wearing gloves, do not touch doorknobs, telephones (also mobiles!), light switches, etc as this will spread contamination. This includes new/clean gloves as well - your colleagues don't know if gloves are clean and could respond unfavourably.
  • Glove types are not equally resistant to all chemicals - use the "right gloves" for the chemical being handled (see chemical SDS's)
  • Change gloves regularly, or immediately in the case of suspected contamination (e.g. Chloroform or Methanol - both penetrate within seconds).
6 Use protective equipment when handling liquid nitrogen (LN2).
7 UV lamps can easily cause burns - protect your skin and eyes and turn off the UV lamp after use.
8 Ask Grethe Underland for the Sars Centre’s Waste Guidelines. Please note the following:
  • Biologically contaminated waste: plastic bag in the metal bin for autoclaving waste (sterilization)
  • Chemically contaminated waste (e.g. Ethidium bromide): yellow box for problem waste (incineration)
  • Organic solvents without halogens: red container
  • Organic solvents with halogens: blue container
  • Inorganic and dangerous chemical waste (e.g. heavy metals): separate collection and disposal in special labelled containers (Registreringsskjema for farlig avfall): red boxes
  • Broken or disposable glassware and sharp materials must be collected in separate containers that are then placed into the yellow box in the storage room. Mark container as ”stikkende/skjærende”.
  • After the use of lab equipment, remove all forms of labeling, rinse the equipment and place it in the designated trays for cleaning.
9 All chemicals, biological cultures, samples, etc. must be marked clearly with contents, name and date in order to avoid incorrect use or destruction of the material.
10

All work with hazardous organic or inorganic solvents must take place in an appropriate fume hood (or equivalently ventilated work station). The green arrow marks the maximal (and also optimal) opening. Opening the hood wider reduces the airflow dramatically. Mind the times with reduced (50%) venting capacity. Ventilation is working on 100% capacity:

  • Weekdays: 07:00 - 19:00 (100% capacity)
  • Weekdays: 19:00 - 07:00 (50% capacity)
  • Saturdays: 09:00-14:00 (100% capacity)
  • Saturdays before 09:00 and after 14:00 (50% capacity)
  • Sundays: All Day only 50%
11 Chemical datasheets (SDS/MSDS) for each chemical must be available in the lab. These provide instructions regarding danger category, handling procedures, waste removal and neutralization of spills. (M)SDS's must be available in Norwegian, but should also be made available in the appropriate language for the given lab. Read the MSDS and inform yourself before using chemicals!
12 In case of chemical exposure to eyes and/or skin, notify your supervisor and ask other colleagues for help. See also Sars Centre's Chemical Exposure Emergency Procedures on Sars Centre's HMS website.
13 Contact persons are appointed for the Sars Centre’s common equipment and rooms (i.e. ultracentrifuge, Sorval centrifuge, autoclave, gel documentation system, confocal microscope, etc.). All new employees must be trained by the contact person before using this equipment. Each employee is responsible for cleaning up after the use of common equipment and rooms. See Sars Common Facilities Responsibility List
14 Ask Marit Flo Jensen (S1) for the Sars Centre’s Guidelines for Working with Isotopes and the required Radiation Safety course.
15 The Cell Lab has restricted access, please ask Simon Henriet (S1) for instructions and access.