General Sars Centre Routines

Sinks and drains

Washing

Glassware and plastic items must be brought to the washing room where they will be cleaned in the dishwasher and returned to the lab group storage cabinets by washroom personnel.

Safety Guidelines:

Autoclaving

There is a common autoclaving service at the Sars Centre for wet and dry autoclaving. Items to be autoclaved should be left in the washroom on the assigned shelves (wet or dry) and will be taken care of by washroom personnel.

Dry Ice

There is a common use storage of dry ice in the -80° ultrafreezer #1. When receiving a shipment which contains dry ice, please add any leftover dry ice to this shared stock.

Safety Guidelines:

UV Radiation - room 234B2

We use an Ultraviolet (UV) Trans-illuminator to visualize fluorescent markers that are used in gel electrophoresis for nucleic acids (DNA, RNA) and SDS-PAGE for proteins. UV trans-illuminators are major sources of UV radiation which can cause damage to your skin and eyes after only very short exposure times.

Risks:

Safety Guidelines:

Liquid Nitrogen (-196 °C)

The Sars Centre has a common supply of liquid nitrogen, located in the corridor adjacent to the -80° freezers.

Risks:

Safety Guidelines:

Gas and Gas cylinders

The Sars Centre currently uses two types of compressed gas:

Nitrogen cylinders (50 litre) MUST ONLY be handled and transported by trained personnel and must be stored in a room specified for the storage of gas under pressure (“Gass under trykk”); such rooms must be clearly labelled.

Propane cylinders (2 or 4 litre) present in most labs, can be used by all lab members after receiving safety and handling instructions.

Propane Safety Guidelines:

To make propane easier to detect in the event of a leak or spill, a chemical compound is added to give it a distinctive smell, like rotten eggs or cabbage. Trust your nose; never ignore the smell of propane. Always treat it as an actual leak until confirmed otherwise.

If you smell propane
  1. No flames or sparks! Immediately put out all open flames. Do not operate telephones, cell phones or other equipment. Do not turn lights on or off. Flames or sparks from these sources can trigger a fire or explosion.
  2. Shut off the gas. If you are able to, safely turn off the cylinder valve. To close the valve, turn it to the right (clockwise).
  3. Report the leak. Leave the area and contact the Safety Delegate and the Head of Administration.

Propane Handling and Storage: