This 10 day intensive course will introduce graduate students and post doctoral researchers to recent molecular and embryological technologies that are applicable on a genome-wide scale in this vertebrate model organism. The course combines high throughput transgenesis using retroviral- as well as transposon vectors with the molecular techniques to rapidly isolate genomic sequence flanking these insertions and mapping them onto the zebrafish genome sequence. The program of this course offers mainly hands-on experience ranging from vector design and -construction to generating and isolating insertions by fluorescence microscopy and relevant embryological and live imaging techniques. The laboratory work will be complemented by lectures on these topics as well as bioinformatics and data management. There will be a one-day theoretical course on Targeting Induced Local Lesions IN Genomes (TILLING). Topics are taught and supervised by local and external teachers who have pioneered these technologies.