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Gene Trapping |
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Koichi Kawakami, National Institute of Genetics, Mishima, Japan | external teacher
kokawaka@lab.nig.ac.jp
Tol2 is a transposable element isolated from the genome of the medaka fish (1).
We found that Tol2 encodes a transposase, which is capable of catalyzing the
transposition reaction. By using the Tol2 transposon system, we developed a highly
efficient transgenesis method in zebrafish. We further we developed a transposon-based
gene trap vector and showed that this construct could entrap endogenous transcripts
in the zebrafish genome and fish expressing GFP in temporally and spatially restricted
patterns could be obtained very efficiently (2).
In this course, we will describe how this transposon system works in zebrafish
and how transposon insertions and the trapped endogenous gene can be characterized,
and will discuss how this transposon technology will be applicable to further
development of genetic methodologies in zebrafish, which should lead to better
understanding of vertebrate development and organogenesis.
Suggested Readings
- (1) Koga, A., Suzuki, M., Inagaki, H., Bessho, Y., and Hori, H. Nature 383:30
(1996).
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(2) Kawakami, K., Takeda, H., Kawakami, N., Kobayashi, M., Matsuda, N., and
Mishina, M. Developmental Cell 7:133-144 (2004).
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