Appendicularia Facility (Chourrout and Thompson)

The Oikopleura dioica culture facility at the Sars centre consists of 46 tanks at 15±0,5°C and 26 tanks at room temperature (19-20°C) allowing at present the production of 7000 mature adults every week (lifecycle≈6 days at 15°C), and the delivery of juveniles at various stages. The facility runs several populations differing in the initial place of collection in the fjords and the number of generations spent in culture conditions. The culture protocol is permanently improved so to reduce the manpower/tank ratio, and ultimately allow the establishment of genetic screens. Work at the facility also contributed to facilitate the manipulation of gametes, embryos and adults, including the establishment of sperm cryopreservation.

Inside Appendic Park
Inside Appendic Park

The culture facility supports research of the Chourrout and Thompson laboratories, as well as external collaborations. External labs are encouraged to contact the facility manager for sending staff or students to learn the detailed culture protocol. Support for travel and accomodation will be provided when required and possible. The facility has also produced material and resources used for molecular biology and genome sequencing (cDNA libraries, BAC libraries, sperm for shotgun sequencing, etc....).

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Sars International Centre for Marine Molecular Biology
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