ANNEX 5: Isotope Room
Contact Person: Marit Flo Jensen
The Norwegian Radiation Protection Authority (Statens Strĺlevern - www.nrpa.no) is the national authority regarding radiation protection and nuclear safety in Norway. Focus is based on international recommendations and Norwegian legislation, in particular the Act on Radiation Protection and Use of Radiation (norsk) and the Regulation to the Act (norsk), and also the Act on Nuclear Energy Activities. The University of Bergen and Uni Research have developed guidelines for work with radioactive nucleotides (norsk).
Brief guidelines for work in the Isotope room
Only authorised personnel are allowed to use the room. Group Leaders are responsible for ensuring the training and authorisation of lab members. For detailed instructions regarding safety, rules and best practices in the Isotope room, please refer to the facility handbook.
- All users must attend the University of Bergen course in radioactivity which is held annually.
- All radioactive material purchases must be approved by Group Leaders. A copy of order or packing document must be given to the isotope contact person.
- A lab coat must be worn at all times.
- Gloves must be worn at all times and removed before leaving the isotope room. For your own protection, it is recommend that you wear 2 sets of gloves.
- PRIOR to beginning work, all users must register in the logbook and control the area for contamination. See detailed instructions in facility handbook: “Duty List (Monitor Test and Wipe Test)”
- All isotopes must be labelled with number, type, amount, storage and usage. Label flasks and tubes with name and lab for easy identification.
- Concentrated stock solutions are only to be opened in the isotope room. Maximum outside the isotope room: 32P: 50 µCi, 35S: 100 µCi .
- Use the radioactivity monitor at all times to keep control of contamination before, during and after work. Spills are easily removed if cleaned immediately.
- Major spills should be taken care of immediately and reported to the contact person. In the case of skin exposure: wash with plenty of cold water and soap, rub for at least three minutes and rinse frequently. See detailed instructions in facility handbook: ”Isotope Accident Procedures”
- 32P must be protected at all times. i.e. when moving tube from bench to centrifuge - move the shield at the same time. Use screen when looking for pellets in eppendorfs - eyes are particularly vulnerable, hold tubes on less radioactive end or better, use forceps.
- Hot solutions are kept in bottles marked with name and date of isotope, and then set for decay. See detailed instructions in facility handbook: “Isotope Waste Procedures”
- Never discard needles, scalpels and glass Pasteur pipettes directly into waste bag for low-radioactivity. Use plastic containers such as empty ethanol bottles, chemical bottles etc. Discard entire container when full. See detailed instructions in facility handbook: “Isotope Waste Procedures”
- When work is complete, clean all areas used, remove your things, perform monitor contamination control, and sign out in the logbook.
- If you move radioactive samples to your ordinary lab, make sure that the containers are labelled with radioactive tape and that you have sufficient shielding against the radiation.
- Currently, users are not required to wear dosimeters when working in the Isotope Room at the Sars Centre.