Genetically modified microorganisms (GMO) and microorganisms (GMM) MUST NEVER be released directly into the environment.
Bacterial waste and genetically modified microorganisms (GMM) waste
Biologically contaminated waste (gloves, paper, tips, plastics, used agar plates) must be discarded in plastic autoclave bags, inside the metal bins found in each lab. This waste will be sterilised by autoclaving and then returned to the lab and they must throw the waste.
Never put chemically contaminated waste in the metal bins - use the yellow boxes.
Liquid bacterial waste is sterilised by autoclaving. It must be placed in an autoclaveable container, filled to an appropriate level, labelled with contents and your initials. Users must deliver these waste containers to the washroom for processing.
70% ethanol can be used tor disinfection of equipment that cannot be autoclaved or exposed to corrosive agents, and also to clean GMM spills on benches.
Genetically modified organisms (GMO) waste
Macroscopic (large animal) transgenic waste - e.g. zebrafish, adult Ciona
- Waste can be temporarily be stored in a designated freezer. It should then be transferred to a yellow box (special/problem waste), properly labelled with content, name and date, and brought to the waste room on HiB 1st floor on the evening before special waste is collected. Ask Grethe Underland for collection schedule.
Microscopic (larvae/small animal) transgenic waste - e.g. Nematostella
- Dispose ONLY in sinks / drains located in animal facilities, which lead to the water purification pyramid (where the waste is chlorinated and irradiated).
- Chemically treated larvae /small animals must NOT go into sinks or drains! They should be disposed in the yellow special waste containers; excess reagents, solutions should be collected and disposed of as hazardous / dangerous waste.
Note: the preceding applies to both stable and transiently transgenic organisms.
- Must be destroyed before releasing into the environment as low concentrations of antibiotics can promote antibiotic resistance in bacteria outside the lab.
- Autoclave liquids or solids as an efficient way of destroying heat labile antibiotics.